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Substrate water exchange in photosystem II depends on the peripheral proteins

Hillier, Warwick; Hendry, G; Burnap, Robert L; Wydrzynski, Thomas

Description

The 18O exchange rates for the substrate water bound in the S3 state were determined in different photosystem II sample types using time-resolved mass spectrometry. The samples included thylakoid membranes, salt-washed Triton X-100-prepared membrane fragments, and purified core complexes from spinach and cyanobacteria. For each sample type, two kinetically distinct isotopic exchange rates could be resolved, indicating that the biphasic exchange behavior for the substrate water is inherent to...[Show more]

CollectionsANU Research Publications
Date published: 2001
Type: Journal article
URI: http://hdl.handle.net/1885/92819
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.M102954200

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