Skip navigation
Skip navigation

Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis

Hulett, Mark; Freeman, Craig; Hamdorf, B; Baker, Rohan; Harris, Matthew; Parish, Christopher

Description

The endoglycosidase heparanase is an important in the degradation of the extracellular matrix by invading cells, notably metastatic tumor cells and migrating leukocytes. Here we report the cDNA sequence of the human platelet enzyme, which encodes a unique protein of 543 amino acids, and the identification of highly homologous sequences in activated mouse T cells and in a highly metastatic rat adenocarcinoma. Furthermore, the expression of heparanase mRNA in rat tumor cells correlates with their...[Show more]

dc.contributor.authorHulett, Mark
dc.contributor.authorFreeman, Craig
dc.contributor.authorHamdorf, B
dc.contributor.authorBaker, Rohan
dc.contributor.authorHarris, Matthew
dc.contributor.authorParish, Christopher
dc.date.accessioned2015-12-13T23:25:06Z
dc.identifier.issn1078-8956
dc.identifier.urihttp://hdl.handle.net/1885/92517
dc.description.abstractThe endoglycosidase heparanase is an important in the degradation of the extracellular matrix by invading cells, notably metastatic tumor cells and migrating leukocytes. Here we report the cDNA sequence of the human platelet enzyme, which encodes a unique protein of 543 amino acids, and the identification of highly homologous sequences in activated mouse T cells and in a highly metastatic rat adenocarcinoma. Furthermore, the expression of heparanase mRNA in rat tumor cells correlates with their metastatic potential. Exhaustive studies have shown only one heparanase sequence, consistent with the idea that this enzyme is the dominant endoglucuronidase in mammalian tissues.
dc.publisherNature Publishing Group
dc.sourceNature Medicine
dc.subjectKeywords: heparanase; adenocarcinoma; article; cancer invasion; DNA sequence; enzyme analysis; gene expression; human; metastasis; molecular cloning; nucleotide sequence; pathogenesis; priority journal; sequence analysis; Adenocarcinoma; Amino Acid Sequence; Animal
dc.titleCloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume5
dc.date.issued1999
local.identifier.absfor111299 - Oncology and Carcinogenesis not elsewhere classified
local.identifier.ariespublicationMigratedxPub23634
local.type.statusPublished Version
local.contributor.affiliationHulett, Mark, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationFreeman, Craig, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHamdorf, B, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBaker, Rohan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHarris, Matthew, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationParish, Christopher, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue7
local.bibliographicCitation.startpage803
local.bibliographicCitation.lastpage809
local.identifier.doi10.1038/10525
dc.date.updated2015-12-12T09:23:20Z
local.identifier.scopusID2-s2.0-0033028789
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Hulett_Cloning_of_mammalian_1999.pdf213.05 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator