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Cell surface exposure of phosphatidylserine in propidium iodide negative thymocytes undergoing cell death by necrosis

Waring, Paul; Lambert, David; Sjaarda, A; Hurne, Alanna; Beaver, Joanne


Phosphatidylserine (PS) exposure on propidium iodide negative cells using FITC labelled annexin-V has been used to quantify apoptosis in vitro and in vivo. Detection of PS with in cells undergoing necrosis is also possible if labelled annexin-V specific for PS enters the cell following early membrane damage. Necrotic or late apoptotic cells can be excluded from flow cytometric analysis using propidium iodide which enters and stains cells with compromised membrane integrity. Here we show that...[Show more]

CollectionsANU Research Publications
Date published: 1999
Type: Journal article
Source: Cell Death and Differentiation


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