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A Method for the Purification and Refolding of a Recombinant Form of the Nontypeable Haemophilus influenzae P5 Outer Membrane Protein Fused to Polyhistidine

Webb, Dianne; Cripps, Allan


Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen, commonly associated with otitis media and exacerbations of chronic bronchitis. Studies concerning the pathogenesis of NTHi have proposed an important function for P5, an outer membrane protein believed to play a role in the initiation of infection by mediating adherence to respiratory mucin. P5 has also generated interest as a potential vaccine candidate. In a previous study, an NTHi library screen with antibodies raised...[Show more]

CollectionsANU Research Publications
Date published: 1999
Type: Journal article
Source: Protein Expression and Purification
DOI: 10.1006/prep.1998.0990


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