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31P and 1H NMR spectroscopic studies of liver extracts of carbon tetrachloride-treated rats

Harvey, Peta; Gready, Jill; Hickey, Haruyo; Le Couteur, D; McLean, Allan J

Description

NMR spectroscopy was used to examine hepatic metabolism in cirrhosis with a particular focus on markers of functional cellular hypoxia. 31P and 1H NMR spectra were obtained from liver extracts from control rats and from rats with carbon tetrachloride-induced cirrhosis. A decrease of 34% in total phosphorus content was observed in cirrhotic rats, parallelling a reduction of 40% in hepatocyte mass as determined by morphometric analysis. Hypoxia appeared to be present in cirrhotic rats, as...[Show more]

dc.contributor.authorHarvey, Peta
dc.contributor.authorGready, Jill
dc.contributor.authorHickey, Haruyo
dc.contributor.authorLe Couteur, D
dc.contributor.authorMcLean, Allan J
dc.date.accessioned2015-12-13T23:24:56Z
dc.identifier.issn0952-3480
dc.identifier.urihttp://hdl.handle.net/1885/92452
dc.description.abstractNMR spectroscopy was used to examine hepatic metabolism in cirrhosis with a particular focus on markers of functional cellular hypoxia. 31P and 1H NMR spectra were obtained from liver extracts from control rats and from rats with carbon tetrachloride-induced cirrhosis. A decrease of 34% in total phosphorus content was observed in cirrhotic rats, parallelling a reduction of 40% in hepatocyte mass as determined by morphometric analysis. Hypoxia appeared to be present in cirrhotic rats, as evidenced by increased inorganic phosphate levels, decreased ATP levels, decreased ATP:ADP ratios (1.72 ± 0.40 vs 2.48 ± 0.50, p < 0.01), and increased inorganic phosphate:ATP ratios (2.77 ± 0.48 vs 1.62 ± 0.24, p < 0.00001). When expressed as a percentage of the total phosphorus content, higher levels of phosphoethanolamine and lower levels of NAD and glycerophosphoethanolamine were detected in cirrhotic rats. Cirrhotic rats also had increased phosphomonoester:phosphodiester ratios (5.73 ± 2.88 vs 2.53 ± 0.52, p < 0.01). These findings are indicative of extensive changes in cellular metabolism in the cirrhotic liver, with many findings attributable to the presence of intracellular hypoxia.
dc.publisherJohn Wiley & Sons Inc
dc.sourceNMR in Biomedicine
dc.subjectKeywords: carbon tetrachloride; adenosine diphosphate; adenosine triphosphate; carbon tetrachloride; phosphorus; proton; tissue extract; animal experiment; animal model; animal tissue; article; cell hypoxia; cell metabolism; liver biopsy; liver cirrhosis; liver met Carbon tetrachloride; Hypoxia; Liver cirrhosis; Nuclear magnetic resonance; Rat
dc.title31P and 1H NMR spectroscopic studies of liver extracts of carbon tetrachloride-treated rats
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume12
dc.date.issued1999
local.identifier.absfor110199 - Medical Biochemistry and Metabolomics not elsewhere classified
local.identifier.absfor110302 - Clinical Chemistry (diagnostics)
local.identifier.ariespublicationMigratedxPub23562
local.type.statusPublished Version
local.contributor.affiliationHarvey, Peta, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGready, Jill, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHickey, Haruyo, University of Sydney
local.contributor.affiliationLe Couteur, D, University of Sydney
local.contributor.affiliationMcLean, Allan J, Canberra Hospital
local.description.embargo2037-12-31
local.bibliographicCitation.startpage395
local.bibliographicCitation.lastpage401
local.identifier.doi10.1002/(SICI)1099-1492(199910)12:6<395::AID-NBM568>3.0.CO;2-M
dc.date.updated2015-12-12T09:22:35Z
local.identifier.scopusID2-s2.0-0032589421
CollectionsANU Research Publications

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