Eager, K; Dulhunty, Angela
The location of reactive cysteine residues on the ryanodine receptor (RyR) calcium release channel was assessed from the changes in channel activity when oxidizing or reducing reagents were added to the luminal or cytoplasmic solution. Single sheep cardiac RyRs were incorporated into lipid bilayers with 10-7 M cytoplasmic Ca2+. The thiol specific-lipophilic- 4,4'-dithiodipyridine (4,4'-DTDP, 1 mM), as well as the hydrophilic thimerosal (1 mM), activated and then inhibited RyRs from either the...[Show more]
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