Immunotherapeutic Potential of Dendritic Cells Generated in Long Term Stroma-Dependent Cultures
Long term cultures (LTC) producing dendritic cells (DC) have been established from spleen. A well developed stromal cell layer supported production of DC in numbers suitable for experimentation. Cells had obvious membrane pseudopodia and could be collected from culture every 2-3 days. Large cells produced in LTC stained with fluorescently labelled monoclonal antibodies specific for DC such as 33D1, and M1/70 which is specific for DC and myeloid cells. These staining patterns confirmed the...[Show more]
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|Source:||Cancer Biotherapy and Radiopharmaceuticals|
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