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Immunotherapeutic Potential of Dendritic Cells Generated in Long Term Stroma-Dependent Cultures

O'Neill, Helen; Jonas, N; Wilson, Heather; Ni, Keping


Long term cultures (LTC) producing dendritic cells (DC) have been established from spleen. A well developed stromal cell layer supported production of DC in numbers suitable for experimentation. Cells had obvious membrane pseudopodia and could be collected from culture every 2-3 days. Large cells produced in LTC stained with fluorescently labelled monoclonal antibodies specific for DC such as 33D1, and M1/70 which is specific for DC and myeloid cells. These staining patterns confirmed the...[Show more]

CollectionsANU Research Publications
Date published: 2000
Type: Journal article
Source: Cancer Biotherapy and Radiopharmaceuticals


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