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A Molecular Mousetrap Determines Polarity of Termination of DNA Replication in E. coli

Mulcair, Mark; Schaeffer, Patrick; Oakley, Aaron; Cross, Hannah F; Neylon, Cameron; Hill, Thomas M; Dixon, Nicholas

Description

During chromosome synthesis in Escherichia coli, replication forks are blocked by Tus bound Ter sites on approach from one direction but not the other. To study the basis of this polarity, we measured the rates of dissociation of Tus from forked TerB oligonucleotides, such as would be produced by the replicative DnaB helicase at both the fork-blocking (nonpermissive) and permissive ends of the Ter site. Strand separation of a few nucleotides at the permissive end was sufficient to force rapid...[Show more]

CollectionsANU Research Publications
Date published: 2006
Type: Journal article
URI: http://hdl.handle.net/1885/91997
Source: Cell
DOI: 10.1016/j.cell.2006.04.040

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