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Comparative metabolism of dietary terpene, p-cymene, in generalist and specialist folivorous marsupials

Boyle, Rebecca; McLean, Stuart; Foley, William; Davies, Noel

Description

The urinary metabolites of the monoterpene, p-cymene, were studied in three marsupial species: a generalist herbivore, the brushtail possum (Trichosurus vulpecula), and two specialist folivores, the greater glider (Petauroides volans) and the ringtail possum (Pseudocheirus peregrinus), as well as in the laboratory rat (Rattus norvegicus). Each animal was dosed, intragastrically, with single doses of p-cymene (0.37 mmol/kg and/or 1.49 mmol/kg). Urine and feces were collected for two 24-hr...[Show more]

dc.contributor.authorBoyle, Rebecca
dc.contributor.authorMcLean, Stuart
dc.contributor.authorFoley, William
dc.contributor.authorDavies, Noel
dc.date.accessioned2015-12-13T23:23:33Z
dc.identifier.issn0098-0331
dc.identifier.urihttp://hdl.handle.net/1885/91842
dc.description.abstractThe urinary metabolites of the monoterpene, p-cymene, were studied in three marsupial species: a generalist herbivore, the brushtail possum (Trichosurus vulpecula), and two specialist folivores, the greater glider (Petauroides volans) and the ringtail possum (Pseudocheirus peregrinus), as well as in the laboratory rat (Rattus norvegicus). Each animal was dosed, intragastrically, with single doses of p-cymene (0.37 mmol/kg and/or 1.49 mmol/kg). Urine and feces were collected for two 24-hr periods. Quantitative analysis of urinary metabolites by extraction, gas chromatography, and mass spectrometry gave a mean recovery of 64% (range 52-74%) of the administered dose in 48 hr for the four species. No fecal metabolites were found. A species-specific pattern of metabolite excretion was evident and reflected the natural occurrence of p-cymene (and terpenes in general) in the diet. If the metabolites excreted are grouped according to the total number of oxygen atoms added (one to four), then the rat excreted metabolites encompassing all degrees of oxidation, but predominantly a monooxygenated metabolite. The brushtail possum excreted metabolites having two, three, and four oxygen atoms added. The ringtail possum and greater glider only excreted metabolites with three or four oxygen atoms. Conjugation played a significant role in the excretion of oxidized metabolites in only the brushtail possums and the rat. These findings indicate that species encountering terpenes, such as p-cymene, in their normal diet have developed efficient oxidation pathways to eliminate them. This oxidative efficiency could also reduce the necessity for subsequent conjugation of metabolites which minimizes further demands on a nutritionally limited diet.
dc.publisherPlenum Publishing Corporation
dc.sourceJournal of Chemical Ecology
dc.subjectKeywords: para cymene; terpene; animal experiment; article; conjugation; controlled study; detoxification; diet; extraction; feces level; gas chromatography; marsupial; mass spectrometry; metabolism; metabolite; nonhuman; opossum; oxidation; quantitative assay; rat Detoxification; Eucalyptus; Metabolism; p- cymene; Petauroides volans; Pseudocheirus peregrinus; Terpene; Trichosurus vulpecula
dc.titleComparative metabolism of dietary terpene, p-cymene, in generalist and specialist folivorous marsupials
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume25
dc.date.issued1999
local.identifier.absfor060603 - Animal Physiology - Systems
local.identifier.ariespublicationMigratedxPub22730
local.type.statusPublished Version
local.contributor.affiliationBoyle, Rebecca, University of Tasmania
local.contributor.affiliationMcLean, Stuart, University of Tasmania
local.contributor.affiliationFoley, William, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationDavies, Noel, University of Cambridge
local.description.embargo2037-12-31
local.bibliographicCitation.issue9
local.bibliographicCitation.startpage2109
local.bibliographicCitation.lastpage2126
dc.date.updated2015-12-12T09:16:50Z
local.identifier.scopusID2-s2.0-0032837110
CollectionsANU Research Publications

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