Linear fidelity in quantification of anti-viral CD8+ T cells
Enumeration of anti-viral CD8+ T cells to make comparisons between mice, viruses and vaccines is a frequently used approach, but controversy persists as to the most appropriate methods. Use of peptide-MHC tetramers (or variants) and intracellular staining for cytokines, in particular IFNγ after a short ex vivo stimulation are now common, as are a variety of cytotoxicity assays, but few direct comparisons have been made. It has been argued that use of tetramers leads to the counting of...[Show more]
|Collections||ANU Research Publications|
|Source:||PLoS ONE 7.6 (2012): e39533|
|Flesch_Linear2012.pdf||411.01 kB||Adobe PDF|
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