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Linear fidelity in quantification of anti-viral CD8+ T cells

Flesch, Inge E. A.; Hollett, Natasha A.; Wong, Yik Chun; Tscharke, David C.


Enumeration of anti-viral CD8+ T cells to make comparisons between mice, viruses and vaccines is a frequently used approach, but controversy persists as to the most appropriate methods. Use of peptide-MHC tetramers (or variants) and intracellular staining for cytokines, in particular IFNγ after a short ex vivo stimulation are now common, as are a variety of cytotoxicity assays, but few direct comparisons have been made. It has been argued that use of tetramers leads to the counting of...[Show more]

CollectionsANU Research Publications
Date published: 2012-06-20
Type: Journal article
Source: PLoS ONE 7.6 (2012): e39533
DOI: 10.1371/journal.pone.0039533


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