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Antigen-specific systemic and reproductive tract antibodies in foxes immunized with salmonella typhimurium expressing bacterial and sperm proteins

Bird, Phillip I; Verma, Naresh; Bradley, Mark P

Description

Attenuated Salmonella typhimurium strains are potential 'safe' delivery vectors of an oral immunocontraceptive vaccine for the European red fox (Vulpes vulpes). In the present study, model bacterial (Escherichia coli heat-labile enterotoxin B subunit, LTB) and fox sperm (fSP10) antigens were expressed in S. typhimurium SL3261 (ΔaroA) under the control of the trc promoter. Adult female foxes were given three oral immunizations with SL3261 containing either LTB (SL3261/pLTB), fSP10...[Show more]

dc.contributor.authorBird, Phillip I
dc.contributor.authorVerma, Naresh
dc.contributor.authorBradley, Mark P
dc.date.accessioned2015-12-13T23:22:38Z
dc.date.available2015-12-13T23:22:38Z
dc.identifier.issn1031-3613
dc.identifier.urihttp://hdl.handle.net/1885/91540
dc.description.abstractAttenuated Salmonella typhimurium strains are potential 'safe' delivery vectors of an oral immunocontraceptive vaccine for the European red fox (Vulpes vulpes). In the present study, model bacterial (Escherichia coli heat-labile enterotoxin B subunit, LTB) and fox sperm (fSP10) antigens were expressed in S. typhimurium SL3261 (ΔaroA) under the control of the trc promoter. Adult female foxes were given three oral immunizations with SL3261 containing either LTB (SL3261/pLTB), fSP10 (SL3261/pFSP10) or a control plasmid (pKK233-2 or pTrc99A). All foxes raised serum (IgG) and vaginal (IgG and IgA) antibodies against S. typhimurium lipopolysaccharide (LPS). Each fox that received SL3261/pLTB raised high titre LTB-specific serum and vaginal IgG antibodies. However, only one of four foxes immunized with SL3261/pFSP10 raised an anti-fSP10 immune response, in the form of low titre serum and vaginal IgG antibodies. No vaginal IgA antibodies were raised against either LTB or fSP10 in these experiments. The immune responses against recombinant LTB and fSP10 resulted chiefly from the initial dose of antigen in the inocula and were minimally influenced by continued in vivo antigen expression. This study demonstrates for the first time in the female red fox that oral Salmonella can elicit specific systemic and reproductive tract antibodies against heterologous, recombinant proteins.
dc.publisherCSLI Publications
dc.sourceReproduction, Fertility and Development
dc.subjectKeywords: bacterium lipopolysaccharide; contraceptive agent; Escherichia coli enterotoxin; immunoglobulin G antibody; sperm antigen; antigen expression; article; drug delivery system; female; fox; immune response; immunocontraception; nonhuman; Salmonella typhimuri Canine; Escherichia coli heat-labile enterotoxin B subunit (LTB); Fox sperm antigen; Immunocontraception; Oral immunization; SP10; Vulpes vulpes
dc.titleAntigen-specific systemic and reproductive tract antibodies in foxes immunized with salmonella typhimurium expressing bacterial and sperm proteins
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume11
dc.date.issued1999
local.identifier.absfor060503 - Microbial Genetics
local.identifier.ariespublicationMigratedxPub22309
local.type.statusPublished Version
local.contributor.affiliationBird, Phillip I, Monash University
local.contributor.affiliationVerma, Naresh, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationBradley, Mark P, CRC for the Biological Control of Pest Animals (CSIRO)
local.bibliographicCitation.startpage219
local.bibliographicCitation.lastpage228
dc.date.updated2015-12-12T09:11:49Z
local.identifier.scopusID2-s2.0-0033503370
CollectionsANU Research Publications

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