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Stimulation of Dengue Virus Replication in Cultured Aedes albopictus (C6/36) Mosquito Cells by the Antifungal Imidazoles Ketoconazole and Miconazole

Lee, Eva; McLean, K; Weir, Ron; Dalgarno, L

Description

Replication of dengue type 3 virus in Aedes albopictus C6/36 cells was enhanced more than 50-fold by addition of the antifungal imidazole derivative ketoconazole within the first 4 h of infection. The stimulatory effect was reflected in the yield of infectious virus and in levels of viral RNA and protein synthesis. Enhanced yields were observed also for other flaviviruses, including dengue type 2 virus and Murray Valley encephalitis virus. Increased yields of dengue type 3 virus were not...[Show more]

dc.contributor.authorLee, Eva
dc.contributor.authorMcLean, K
dc.contributor.authorWeir, Ron
dc.contributor.authorDalgarno, L
dc.date.accessioned2015-12-13T23:21:05Z
dc.identifier.issn0042-6822
dc.identifier.urihttp://hdl.handle.net/1885/91015
dc.description.abstractReplication of dengue type 3 virus in Aedes albopictus C6/36 cells was enhanced more than 50-fold by addition of the antifungal imidazole derivative ketoconazole within the first 4 h of infection. The stimulatory effect was reflected in the yield of infectious virus and in levels of viral RNA and protein synthesis. Enhanced yields were observed also for other flaviviruses, including dengue type 2 virus and Murray Valley encephalitis virus. Increased yields of dengue type 3 virus were not observed in African green monkey kidney (Vero) cells, human monocytic (U-937) cells, or cells of the mosquito Toxorhynchites amboinensis (TRA-171). (C) 2000 Academic Press.
dc.publisherAcademic Press
dc.sourceVirology
dc.subjectKeywords: ketoconazole; miconazole; virus RNA; Aedes albopictus; animal cell; article; controlled study; Dengue virus; human; human cell; Japanese encephalitis virus; monocyte; mosquito; nonhuman; priority journal; protein synthesis; Vero cell; virus replication; A
dc.titleStimulation of Dengue Virus Replication in Cultured Aedes albopictus (C6/36) Mosquito Cells by the Antifungal Imidazoles Ketoconazole and Miconazole
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume269
dc.date.issued2000
local.identifier.absfor110804 - Medical Virology
local.identifier.ariespublicationMigratedxPub21520
local.type.statusPublished Version
local.contributor.affiliationLee, Eva, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMcLean, K, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWeir, Ron, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationDalgarno, L, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage6
local.identifier.doi10.1006/viro.2000.0217
dc.date.updated2015-12-12T09:05:26Z
local.identifier.scopusID2-s2.0-0034732321
CollectionsANU Research Publications

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