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Limited proteolysis as a powerful tool for probing flexible oligopeptide portions among different classes of glutathione transferases

Nuccetelli, Marzia; Evangelisti, Cristina; Capo, Concetta; Petruzzelli, Raffaele; Parker, Michael William; Board, Philip; Caccuri, Anna Maria; Federici, Giorgio; Ricci, G; Lo Bello, Mario


We have probed possible flexible regions of different GSTs by limited proteolysis experiments. The results indicate that each isoenzyme is inactivated by trypsin and protected against this proteolytic attack by glutathione (GSH). Unlike the results previously obtained with glutathione transferases (GST) GST P1-1, the region most susceptible to the cleavage and responsible for the loss of enzymatic activity in GST A1-1 is located at C-terminus (helix 9) whilst GST M2-2 does not show any primary...[Show more]

CollectionsANU Research Publications
Date published: 2001
Type: Journal article
Source: Chemico-Biological Interactions


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