The Escherichia coli signal transducers PII (GlnB) and GlnK form heterotrimers in vivo: Fine tuning the nitrogen signal cascade
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Van Heeswijk, W; Wen, Daying; Clancy, Paula; Jaggi, Rene; Ollis, David; Westerhoff, Hans; Vasudevan, Subhash
Description
The PII protein is Escherichia coli's cognate transducer of the nitrogen signal to the NRII (NtrB)/NRI (NtrC) two-component system and to adenylyltransferase. Through these two routes, PII regulates both amount and activity of glutamine synthetase. GInK is the recently discovered paralogue of PII, with a similar trimeric x-ray structure. Here we show that PII and GlnK form heterotrimers, in E. coli grown in nitrogen-poor medium. In vitro, fully uridylylated heterotrimers of the two proteins...[Show more]
Collections | ANU Research Publications |
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Date published: | 2000 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/89993 |
Source: | PNAS - Proceedings of the National Academy of Sciences of the United States of America |
DOI: | 10.1073/pnas.97.8.3942 |
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01_Van Heeswijk_The_Escherichia_coli_signal_2000.pdf | 147.64 kB | Adobe PDF | Request a copy |
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