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Improved FACS analysis confirms generation of immature dendritic cells in long-term stromal-dependent spleen cultures

Ni, Keping; O'Neill, Helen


Cells produced in spleen stroma-dependent long-term cultures (LTC) have now been clearly defined as dendritic cells (DC). Characterization of cells by antibody staining and FACS analysis has only been possible using a procedure to quench the high autofluorescence of DC produced in LTC (LTC-DC). The population of large cells produced by the established LTC-X1 culture are homogeneously positive for a number of cell-surface markers expressed by DC. These include CD11c, CD11b, Dec-205, Fc receptor...[Show more]

CollectionsANU Research Publications
Date published: 2000
Type: Journal article
Source: Immunology and Cell Biology
DOI: 10.1046/j.1440-1711.2000.00897.x


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