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Scanning Transmission Ion Microscopy Tomography at the Leipzig Nanoprobe LIPSION

Reinert, Tilo; Sakellariou, Arthur; Schwertner, Michael; Vogt, J; Butz, Tilman

Description

Scanning transmission ion microscopy (STIM) of joint cartilage could visualise single collagen fibrils. Thus, answers to the controversial questions of their alignment could be given. However, the fibrils form three-dimensional structures that are not yet fully disclosed. STIM tomography is needed to give more detailed information. The size of the structures requires a challenging resolution of about 100 nm. The first STIM tomographic experiment has been performed at the Leipzig nanoprobe...[Show more]

dc.contributor.authorReinert, Tilo
dc.contributor.authorSakellariou, Arthur
dc.contributor.authorSchwertner, Michael
dc.contributor.authorVogt, J
dc.contributor.authorButz, Tilman
dc.date.accessioned2015-12-13T23:16:50Z
dc.identifier.issn0168-583X
dc.identifier.urihttp://hdl.handle.net/1885/89604
dc.description.abstractScanning transmission ion microscopy (STIM) of joint cartilage could visualise single collagen fibrils. Thus, answers to the controversial questions of their alignment could be given. However, the fibrils form three-dimensional structures that are not yet fully disclosed. STIM tomography is needed to give more detailed information. The size of the structures requires a challenging resolution of about 100 nm. The first STIM tomographic experiment has been performed at the Leipzig nanoprobe LIPSION. 360 projections of a cartilage sample (30 μm × 32 μm × 10 μm) were taken. The pixel resolution was 250 × 250 pixels for each projection. The data set was reconstructed at MARC Melbourne using the backprojection of filtered projections technique. The data show the feasibility of STIM tomography in cartilage research. However, experimental inaccuracies (rotational displacement and magnetic stray fields) have limited the resolution thus far. Improvements in the experimental set-up will lead to higher resolution.
dc.publisherElsevier
dc.sourceNuclear Instruments and Methods in Physics Research: Section B
dc.subjectKeywords: Collagen; Data structures; Image reconstruction; Ion beams; Microscopic examination; Visualization; Scanning transmission ion microscopy (STIM); Tomography Cartilage; Collagen; Ion microbeam; Scanning transmission ion microscopy; Subcellular; Tomography
dc.titleScanning Transmission Ion Microscopy Tomography at the Leipzig Nanoprobe LIPSION
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume190
dc.date.issued2002
local.identifier.absfor029904 - Synchrotrons; Accelerators; Instruments and Techniques
local.identifier.ariespublicationMigratedxPub19670
local.type.statusPublished Version
local.contributor.affiliationReinert, Tilo, University of Leipzig
local.contributor.affiliationSakellariou, Arthur, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationSchwertner, Michael, University of Leipzig
local.contributor.affiliationVogt, J, University of Leipzig
local.contributor.affiliationButz, Tilman, University of Leipzig
local.description.embargo2037-12-31
local.bibliographicCitation.startpage266
local.bibliographicCitation.lastpage270
local.identifier.doi10.1016/S0168-583X(01)01186-7
dc.date.updated2015-12-12T08:49:54Z
local.identifier.scopusID2-s2.0-0036570017
CollectionsANU Research Publications

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