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Interaction of the Escherichia coli Replication Terminator Protein (Tus) with DNA: A Model Derived from DNA-Binding Studies of Mutant Proteins by Surface Plasmon Resonance

Neylon, David Cameron; Brown, Susan Elizabeth; Kralicek, Andrew V; Miles, Caroline; Love, Christopher; Dixon, Nicholas


The Escherichia coli replication terminator protein (Tus) binds tightly and specifically to termination sites such as TerB in order to halt DNA replication. To better understand the process of Tus-TerB interaction, an assay based on surface plasmon resonance was developed to allow the determination of the equilibrium dissociation constant of the complex (K(D)) and association and dissocation rate constants for the interaction between Tus and various DNA sequences, including TerB,...[Show more]

CollectionsANU Research Publications
Date published: 2000
Type: Journal article
Source: Biochemistry
DOI: 10.1021/bi001174w


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