Browne, Kylie; Johnstone, Rufus A; Jans, David A; Trapani, Joseph A
We used yeast two-hybrid screening to identify the cytoskeletal protein filamin as a ligand for the proapoptotic protease granzyme B, produced by cytotoxic T lymphocytes. Filamin was directly cleaved by granzyme B when target cells were exposed to granzyme B and the lytic protein perforin, but it was also cleaved in a caspase-dependent manner following the ligation of Fas receptors. A similar pattern of filamin cleavage to polypeptides of ∼110 and 95 kDa was observed in Jurkat cells killed by...[Show more]
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