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Cryo-scanning electron microscopy observations of vessel content durng transpiration in walnut petioles. Facts or artefacts

Canny, Martin; McCully, Margaret; Huang, Cheng


The reliability of the cryo-SEM technique for stabilizing and quantifying embolisms in vessels of transpiring plants has recently been criticized, on the grounds that the embolisms observed are artefacts of the freezing. One of the tests used was a comparison of the embolisms found in rachises of transpiring walnut leaves (Juglans regia L.) frozen intact on the tree, with both cryo-SEM images of vessel contents and the measured hydraulic conductivity of similar samples whose xylem pressure had...[Show more]

CollectionsANU Research Publications
Date published: 2001
Type: Journal article
Source: Plant Physiology and Biochemistry
DOI: 10.1016/S0981-9428(01)01288-8


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