Identification of nolR-regulated proteins in Sinorhizobium meliloti using proteome analysis.
Chen, Hancai; Higgins, Jody; Kondorosi, Eva; Kondorosi, Adam; Djordjevic, Michael; Weinman, Jeremy; Rolfe, Barry
Description
Extractable proteins from Sinorhizobium meliloti strains AK631 and EK698 (a Tn5-induced noIR-deficient mutant of AK631), grown in tryptone agar (TA) medium with or without the addition of the plant signal luteolin, were separated by two-dimensional gel electrophoresis and compared. Analysis of silver-stained gels showed that the noIR mutant had 189 proteins that were significantly altered in their levels (101 protein spots up- and 88 downregulated). Coomassie-stained preparative two-dimensional...[Show more]
Collections | ANU Research Publications |
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Date published: | 2000 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/89123 |
Source: | Electrophoresis |
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