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Identification of nolR-regulated proteins in Sinorhizobium meliloti using proteome analysis.

Chen, Hancai; Higgins, Jody; Kondorosi, Eva; Kondorosi, Adam; Djordjevic, Michael; Weinman, Jeremy; Rolfe, Barry

Description

Extractable proteins from Sinorhizobium meliloti strains AK631 and EK698 (a Tn5-induced noIR-deficient mutant of AK631), grown in tryptone agar (TA) medium with or without the addition of the plant signal luteolin, were separated by two-dimensional gel electrophoresis and compared. Analysis of silver-stained gels showed that the noIR mutant had 189 proteins that were significantly altered in their levels (101 protein spots up- and 88 downregulated). Coomassie-stained preparative two-dimensional...[Show more]

CollectionsANU Research Publications
Date published: 2000
Type: Journal article
URI: http://hdl.handle.net/1885/89123
Source: Electrophoresis

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