The affinities for the two substrate water binding sites in the O2 evolving complex of Photosystem II vary independently during S-state turnover.
The first determinations of substrate water binding to the O2 evolving complex in photosystem II as a complete function of the S states have been made. H218O was rapidly injected into spinach thylakoid samples preset in either the S0, S1, S2, or S3 states, and the rate of 18O incorporation into the O2 produced was determined by time-resolved mass spectrometry. For measurements at m/e = 34 (i.e., for the 16O18O product), the rate of 18O incorporation in all S states shows biphasic kinetics,...[Show more]
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