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An automated fluorescence based assay of neurite formation.

Crouch, Michael F


The assessment of neurite-promoting activity of growth factors and neurotrophins in vitro is usually done by the laborious process of counting neuronal processes manually under a standard microscope. This often requires fixation of the cells so that samples can be counted over a period of days when large numbers of cells or samples are assessed. This, therefore, limits the investigator to a single time point for that plate of cells. In an effort to provide an assay that could be used to screen...[Show more]

CollectionsANU Research Publications
Date published: 2000
Type: Journal article
Source: Journal of Neuroscience Methods
DOI: 10.1016/S0165-0270(00)00328-9


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