An automated fluorescence based assay of neurite formation.
The assessment of neurite-promoting activity of growth factors and neurotrophins in vitro is usually done by the laborious process of counting neuronal processes manually under a standard microscope. This often requires fixation of the cells so that samples can be counted over a period of days when large numbers of cells or samples are assessed. This, therefore, limits the investigator to a single time point for that plate of cells. In an effort to provide an assay that could be used to screen...[Show more]
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|Source:||Journal of Neuroscience Methods|
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