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The Gene for a Novel Member of the Whey Acidic Protein Family Encodes Three Four-disulfide Core Domains and Is Asynchronously Expressed during Lactation

Simpson, Kaylene; Ranganathan, Shoba; Fisher, Juliet; Janssens, Peter; Shaw, Denis; Nicholas, K R


Secretion of whey acidic protein (WAP) in milk throughout lactation has previously been reported for a limited number of species, including the mouse, rat, rabbit, camel, and pig. We report here the isolation of WAP from the milk of a marsupial, the tammar wallaby (Macropus eugenii). Tammar WAP (tWAP) was isolated by reverse-phase HPLC and migrates in SDS-polyacrylamide gel electrophoresis at 29.9 kDa. tWAP is the major whey protein, but in contrast to eutherians, secretion is asynchronous and...[Show more]

CollectionsANU Research Publications
Date published: 2000
Type: Journal article
Source: Journal of Biological Chemistry
DOI: 10.1074/jbc.M002161200


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