Resistance of photoreceptors in the C57BL/6-c2J, C57BL/6J, and BALB/cJ mouse strains to oxygen stress: Evidence of an oxygen phenotype
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Walsh, Natalie; Bravo-Nuevo, A; Stone, Jonathan
Description
Purpose. To assess the vulnerability of retinal photoreceptors in the BALB/cJ, C57BL/6J, and C57BL/6-c2J (c2J) mouse strains to hypoxic and hyperoxic stress. Methods. Mice were raised in dim cyclic light. Pups aged postnatal day 7 (P7) were exposed to hypoxia (11-12% oxygen) for periods up to 23 days. Adult mice were exposed to either hypoxia (12% oxygen) or to hyperoxia (75% oxygen) for up to 2 weeks. Using the TUNEL (terminal dUTP-mediated nick end labeling) technique retinas were examined...[Show more]
dc.contributor.author | Walsh, Natalie | |
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dc.contributor.author | Bravo-Nuevo, A | |
dc.contributor.author | Stone, Jonathan | |
dc.date.accessioned | 2015-12-13T23:14:38Z | |
dc.identifier.issn | 0271-3683 | |
dc.identifier.uri | http://hdl.handle.net/1885/88712 | |
dc.description.abstract | Purpose. To assess the vulnerability of retinal photoreceptors in the BALB/cJ, C57BL/6J, and C57BL/6-c2J (c2J) mouse strains to hypoxic and hyperoxic stress. Methods. Mice were raised in dim cyclic light. Pups aged postnatal day 7 (P7) were exposed to hypoxia (11-12% oxygen) for periods up to 23 days. Adult mice were exposed to either hypoxia (12% oxygen) or to hyperoxia (75% oxygen) for up to 2 weeks. Using the TUNEL (terminal dUTP-mediated nick end labeling) technique retinas were examined for cell death. Results. In juvenile mice, hypoxia induced a robust increase in photoreceptor death in the C57BL/6J strain and a weaker increase in the C57BL/6-c2J strains. In the adult, hypoxia was associated with a small reduction in photoreceptor death in the C57BL/6-c2J strains. Hyperoxia caused substantial photoreceptor death in both the C57BL/6-c2J and C57BL/6J strains. The BALB/cJ strain was more resistant to oxygen stress than the C57BL strains. Conclusions. The difference in oxygen vulnerability between C57BL/6J and BALB/c strains may provide a useful starting point for the analysis of genetic regulation of this vulnerability. The resistance of the C57BL/6-c2J substrains to hypoxia may reflect their degenerative status. | |
dc.publisher | Taylor & Francis Group | |
dc.source | Current Eye Research | |
dc.subject | Keywords: oxygen; animal cell; animal experiment; animal tissue; article; cell death; controlled study; hyperoxia; hypoxia; mouse; mouse strain; nick end labeling; nonhuman; phenotype; photoreceptor; postnatal development; priority journal; Animals; Animals, Newbor Hyperoxia; Hypoxia; Oxygen toxicity; Photoreceptor death | |
dc.title | Resistance of photoreceptors in the C57BL/6-c2J, C57BL/6J, and BALB/cJ mouse strains to oxygen stress: Evidence of an oxygen phenotype | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 29 | |
dc.date.issued | 2004 | |
local.identifier.absfor | 111303 - Vision Science | |
local.identifier.ariespublication | MigratedxPub18504 | |
local.type.status | Published Version | |
local.contributor.affiliation | Walsh, Natalie, University of Sydney | |
local.contributor.affiliation | Bravo-Nuevo, A, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Stone, Jonathan, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 441 | |
local.bibliographicCitation.lastpage | 447 | |
local.identifier.doi | 10.1080/02713680490522416 | |
dc.date.updated | 2015-12-12T08:39:42Z | |
local.identifier.scopusID | 2-s2.0-12344302483 | |
Collections | ANU Research Publications |
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