Complete Spectra of the Far-red Chemiluminescence of the Oxygenase Reaction of Mn 2+ -activated Ribulose-bisphosphate Carboxylase/Oxygenase Establish Excited Mn 2+ as the Source
Chemiluminescence emitted by Mn2+-activated ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) while catalyzing oxygenation was analyzed to clarify the source of the emission. Using dual detectors capturing radiation over a wide range of visible and infrared wavelengths, we tested for radiation from singlet O2 decay and found it to be essentially absent (less than 0.1% of the total luminescence intensity). Spectra were determined between 647 and 885 nm with a very sensitive,...[Show more]
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|Source:||Journal of Biological Chemistry|
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