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Interactions between Charged Amino Acid Residues within Transmembrane Helices in the Sulfate Transporter SHST1

Shelden, Megan; Loughlin, Patrick; Tierney, Mary (Louise); Howitt, Susan


The aim of this study was to identify charged amino acid residues important for activity of the sulfate transporter SHST1. We mutated 10 charged amino acids in or near proposed transmembrane helices and expressed the resulting mutants in a sulfate transport-deficient yeast strain. Mutations affecting four residues resulted in a complete loss of sulfate transport; these residues were D107 and D122 in helix 1 and R354 and E366 in helix 8. All other mutants showed some reduction in transport...[Show more]

CollectionsANU Research Publications
Date published: 2003
Type: Journal article
Source: Biochemistry
DOI: 10.1021/bi034827s


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