Molecular cloning and expression of the dihydrofolate reductase ( DHFR ) gene from adult buffalo fly ( Haematobia irritans exigua ): effects of antifolates
Elvin, Christopher; Liyou, Nancy; Pearson, Roger; Kemp, D; Dixon, Nicholas
Description
The folate analogues methotrexate, aminopterin and pyrimethamine were toxic when fed in a blood meal to adult buffalo flies (Haematobia irritans exigua), but aminopterin caused greater mortality than methotrexate, while trimethoprim was not toxic to adult flies. This is the first recorded instance of mortality in adult insects caused by ingestion of folate analogues. In order to investigate the mechanism of this toxicity, the dihydrofolate reductase (DHFR) gene was cloned from adult buffalo fly...[Show more]
Collections | ANU Research Publications |
---|---|
Date published: | 2003 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/88368 |
Source: | Insect Molecular Biology |
DOI: | 10.1046/j.1365-2583.2003.00399.x |
Download
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.
Updated: 20 July 2017/ Responsible Officer: University Librarian/ Page Contact: Library Systems & Web Coordinator