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Molecular cloning and expression of the dihydrofolate reductase ( DHFR ) gene from adult buffalo fly ( Haematobia irritans exigua ): effects of antifolates

Elvin, Christopher; Liyou, Nancy; Pearson, Roger; Kemp, D; Dixon, Nicholas


The folate analogues methotrexate, aminopterin and pyrimethamine were toxic when fed in a blood meal to adult buffalo flies (Haematobia irritans exigua), but aminopterin caused greater mortality than methotrexate, while trimethoprim was not toxic to adult flies. This is the first recorded instance of mortality in adult insects caused by ingestion of folate analogues. In order to investigate the mechanism of this toxicity, the dihydrofolate reductase (DHFR) gene was cloned from adult buffalo fly...[Show more]

CollectionsANU Research Publications
Date published: 2003
Type: Journal article
Source: Insect Molecular Biology
DOI: 10.1046/j.1365-2583.2003.00399.x


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