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c-Rel Is Required for Chromatin Remodeling Across the IL-2 Gene Promoter

Rao, Sudha; Gerondakis, Steve; Woltring, Donna; Shannon, M Frances

Description

IL-2 gene transcription occurs in an activation-dependent manner in T cells responding to TCR and CD28 activation. One of the critical events leading to increased IL-2 transcription is an alteration in chromatin structure across the 300-bp promoter region of the gene. We initially showed that IL-2 gene transcription in CD4+ primary T cells is dependent on the NF-κB family member, c-Rel, but not RelA. We found that c-Rel is essential for global changes in chromatin structure across the 300-bp...[Show more]

dc.contributor.authorRao, Sudha
dc.contributor.authorGerondakis, Steve
dc.contributor.authorWoltring, Donna
dc.contributor.authorShannon, M Frances
dc.date.accessioned2015-12-13T23:13:36Z
dc.date.available2015-12-13T23:13:36Z
dc.identifier.issn0022-1767
dc.identifier.urihttp://hdl.handle.net/1885/88208
dc.description.abstractIL-2 gene transcription occurs in an activation-dependent manner in T cells responding to TCR and CD28 activation. One of the critical events leading to increased IL-2 transcription is an alteration in chromatin structure across the 300-bp promoter region of the gene. We initially showed that IL-2 gene transcription in CD4+ primary T cells is dependent on the NF-κB family member, c-Rel, but not RelA. We found that c-Rel is essential for global changes in chromatin structure across the 300-bp IL-2 promoter in response to CD3/CD28 in primary CD4+ T cells, but not in response to pharmacological signals, paralleling the requirement for c-Rel in IL-2 mRNA and protein accumulation. Interestingly, measurement of activation-induced localized accessibility changes using restriction enzyme digestion revealed that accessibility close to the c-Rel binding site in the CD28RR region of the promoter is specifically dependent on c-Rel. In contrast, restriction enzyme sites located at a distance from the CD28RR behave independently of c-Rel. These results suggest a nonredundant role for c-Rel in generating a correctly remodeled chromatin state across the IL-2 promoter and imply that the strength of the signal determines the requirement for c-Rel.
dc.publisherAmerican Association of Immunologists
dc.sourceJournal of Immunology
dc.subjectKeywords: CD28 antigen; immunoglobulin enhancer binding protein; interleukin 2; restriction endonuclease; T lymphocyte receptor; transcription factor Rel; transcription factor RelA; unclassified drug; animal cell; article; binding site; chromatin; chromatin structu
dc.titlec-Rel Is Required for Chromatin Remodeling Across the IL-2 Gene Promoter
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume170
dc.date.issued2003
local.identifier.absfor060199 - Biochemistry and Cell Biology not elsewhere classified
local.identifier.ariespublicationMigratedxPub17781
local.type.statusPublished Version
local.contributor.affiliationRao, Sudha, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGerondakis, Steve, Walter and Eliza Hall Institute of Medical Research
local.contributor.affiliationWoltring, Donna, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationShannon, M Frances, College of Medicine, Biology and Environment, ANU
local.bibliographicCitation.startpage3724
local.bibliographicCitation.lastpage3731
dc.date.updated2015-12-12T08:34:58Z
local.identifier.scopusID2-s2.0-0346195644
CollectionsANU Research Publications

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