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Proteome analysis of male gametophyte development in rice anthers

Kerim, Tursun; Imin, Nijat; Weinman, Jeremy; Rolfe, Barry

Description

We used proteomic analysis to investigate the changing patterns of protein synthesis during pollen development in anthers from rice plants grown under strictly controlled growth conditions. Cytological analysis and external growth measurements such as anther length, auricle distances and days before flowering were used to determine pollen developmental stages. This allowed the collection of synchronous anther materials representing six discrete pollen developmental stages. Proteins were...[Show more]

dc.contributor.authorKerim, Tursun
dc.contributor.authorImin, Nijat
dc.contributor.authorWeinman, Jeremy
dc.contributor.authorRolfe, Barry
dc.date.accessioned2015-12-13T23:12:33Z
dc.date.available2015-12-13T23:12:33Z
dc.identifier.issn1615-9853
dc.identifier.urihttp://hdl.handle.net/1885/88113
dc.description.abstractWe used proteomic analysis to investigate the changing patterns of protein synthesis during pollen development in anthers from rice plants grown under strictly controlled growth conditions. Cytological analysis and external growth measurements such as anther length, auricle distances and days before flowering were used to determine pollen developmental stages. This allowed the collection of synchronous anther materials representing six discrete pollen developmental stages. Proteins were extracted from the anther samples and separated by two-dimensional gel electrophoresis to produce proteome maps. The anther proteome maps of different developmental stages were compared and 150 protein spots, which were changed consistently during development, were analysed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry to produce peptide mass fingerprint (PMF) data. Database searches using these PMF data revealed the identities of 40 of the protein spots analyzed. These 40 proteins represent 33 unique gene products. Four protein spots that could not be identified by PMF analysis were analysed by N-terminal microsequencing. Multiple charge-isoforms of vacuolar acid invertase, fructokinase, β-expansin and profilin were identified. These proteins are closely associated with sugar metabolism, cell elongation and cell expansion, all of which are cell activities that are essential to pollen germination. The existence of multiple isoforms of the same proteins suggests that during the process of pollen development some kind of post-translational modification of these proteins occurs.
dc.publisherWiley-VCH Verlag GMBH
dc.sourceProteomics
dc.subjectKeywords: beta fructofuranosidase; fructokinase; patulin; profilin; amino acid sequence; amino terminal sequence; anther; article; carbohydrate metabolism; cell growth; cell kinetics; controlled study; cytology; developmental biology; developmental stage; ear; game Microspore development; Peptide mass fingerprinting-; Rice (Oryza sativa L.); Two-dimensional gel electrophoresis
dc.titleProteome analysis of male gametophyte development in rice anthers
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume3
dc.date.issued2003
local.identifier.absfor060111 - Signal Transduction
local.identifier.ariespublicationMigratedxPub17643
local.type.statusPublished Version
local.contributor.affiliationKerim, Tursun, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationImin, Nijat, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWeinman, Jeremy, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRolfe, Barry, College of Medicine, Biology and Environment, ANU
local.bibliographicCitation.issue5
local.bibliographicCitation.startpage738
local.bibliographicCitation.lastpage751
local.identifier.doi10.1002/pmic.200300424
dc.date.updated2015-12-12T08:32:00Z
local.identifier.scopusID2-s2.0-0038216619
CollectionsANU Research Publications

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