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Effect of early cold stress on the maturation of rice anthers

Imin, Nijat; Kerim, Tursun; Rolfe, Barry; Weinman, Jeremy

Description

Male reproductive development in rice (Oryza sativa Linnaeus is very sensitive to various forms of environmental stresses including low temperature. Here, we present our findings on the proteomic analysis of the later developmental consequences of low temperature treatment on rice anthers. Anther proteins at the trinucleate stage, with or without cold treatment for four days at 12°C at the young microspore stage, were extracted, separated by two-dimensional gel electrophoresis (2-DE) and...[Show more]

dc.contributor.authorImin, Nijat
dc.contributor.authorKerim, Tursun
dc.contributor.authorRolfe, Barry
dc.contributor.authorWeinman, Jeremy
dc.date.accessioned2015-12-13T23:10:05Z
dc.date.available2015-12-13T23:10:05Z
dc.identifier.issn1615-9853
dc.identifier.urihttp://hdl.handle.net/1885/87290
dc.description.abstractMale reproductive development in rice (Oryza sativa Linnaeus is very sensitive to various forms of environmental stresses including low temperature. Here, we present our findings on the proteomic analysis of the later developmental consequences of low temperature treatment on rice anthers. Anther proteins at the trinucleate stage, with or without cold treatment for four days at 12°C at the young microspore stage, were extracted, separated by two-dimensional gel electrophoresis (2-DE) and compared. More than 3000 rice anther proteins of cold-sensitive cultivar Doongara plants at the trinucleate stage were resolved on 2-DE gels over a pH range of 4-7 and detected by silver-staining. Seventy protein spots were differentially displayed after four days of cold treatment at the young microspore stage. Of these, 12 protein spots were newly-induced, 47 were up-regulated, and 11 were down-regulated by cold treatment at the early microspore stage. We identified 18 by matrix-assisted laser desorption/ionization mass spectrometry time of flight (MALDI-TOF) analysis. Of the identified proteins, seven were observed as breakdown (cleavage) products by a combination of 2-DE and MALDI-TOF analysis, thus demonstrating for the first time that cold temperature stress at the young microspore stage enhances and induces partial degradation of proteins in the rice anthers at the trinucleate stage.
dc.publisherWiley-VCH Verlag GMBH
dc.sourceProteomics
dc.subjectKeywords: amino acid sequence; anther; cold stress; cold treatment; conference paper; controlled study; cultivar; low temperature; matrix assisted laser desorption ionization time of flight mass spectrometry; microspore; nonhuman; nucleotide sequence; pH; plant rep Cold-induced male sterility; Peptide mass fingerprint; Pollen development; Rice (Oryza sativa L.); Two-dimensional gel electrophoresis
dc.titleEffect of early cold stress on the maturation of rice anthers
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume4
dc.date.issued2004
local.identifier.absfor060111 - Signal Transduction
local.identifier.ariespublicationMigratedxPub16516
local.type.statusPublished Version
local.contributor.affiliationImin, Nijat, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationKerim, Tursun, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRolfe, Barry, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWeinman, Jeremy, College of Medicine, Biology and Environment, ANU
local.bibliographicCitation.startpage1873
local.bibliographicCitation.lastpage1882
local.identifier.doi10.1002/pmic.200300738
dc.date.updated2015-12-12T08:22:18Z
local.identifier.scopusID2-s2.0-3042771394
CollectionsANU Research Publications

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