Functional Properties of Threefold and Fourfold Channels in Ferritin Deduced from Electrostatic Calculations
Description
The iron storage protein ferritin contains threefold and fourfold symmetric channels that are thought to provide pathways for the transfer of Fe2+ ions in and out of the protein. Using the known crystal structure of the ferritin protein, we perform electrostatic potential energy calculations to elucidate the functional properties of these channels. The threefold channel is shown to be responsible for the transit of Fe2+ ions. Monovalent ions can also diffuse through the threefold channel but...[Show more]
dc.contributor.author | Takahashi, T | |
---|---|---|
dc.contributor.author | Kuyucak, Serdar | |
dc.date.accessioned | 2015-12-13T23:09:48Z | |
dc.identifier.issn | 0006-3495 | |
dc.identifier.uri | http://hdl.handle.net/1885/87165 | |
dc.description.abstract | The iron storage protein ferritin contains threefold and fourfold symmetric channels that are thought to provide pathways for the transfer of Fe2+ ions in and out of the protein. Using the known crystal structure of the ferritin protein, we perform electrostatic potential energy calculations to elucidate the functional properties of these channels. The threefold channel is shown to be responsible for the transit of Fe2+ ions. Monovalent ions can also diffuse through the threefold channel but presence of divalent ions in the pore retards this process leading to a selectivity mechanism similar to the one observed in calcium channels. The fourfold channel is found to be impermeant to all cations with the possible exception of protons. Because proton transfer is essential to maintain the electroneutrality of the protein during iron deposition, we suggest that the function of the fourfold channel is to form a "proton wire" that facilitates their transfer in and out of ferritin. | |
dc.publisher | Biophysical Society | |
dc.source | Biophysical Journal | |
dc.subject | Keywords: calcium channel; ferritin; article; calculation; crystal structure; diffusion coefficient; electricity; energy; iron transport; protein analysis; protein folding; proton transport; transport kinetics; Computer Simulation; Crystallography; Electrostatics; | |
dc.title | Functional Properties of Threefold and Fourfold Channels in Ferritin Deduced from Electrostatic Calculations | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 84 | |
dc.date.issued | 2003 | |
local.identifier.absfor | 029901 - Biological Physics | |
local.identifier.ariespublication | MigratedxPub16331 | |
local.type.status | Published Version | |
local.contributor.affiliation | Takahashi, T, Okazaki National Research Institute | |
local.contributor.affiliation | Kuyucak, Serdar, College of Physical and Mathematical Sciences, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 2256 | |
local.bibliographicCitation.lastpage | 2263 | |
dc.date.updated | 2015-12-12T08:20:46Z | |
local.identifier.scopusID | 2-s2.0-0037380857 | |
Collections | ANU Research Publications |
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