A Mak-like kinase is a repressor of GAMYB in barley aleurone
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Woodger, Fiona; Gubler, Frank; Jacobsen, John; Pogson, Barry
Description
GAMYB is a gibberellin (GA)-regulated activator of hydrolase gene expression in the aleurone layer of germinating cereal grains. Although it is clear that GAMYB expression is regulated by GA, more remains to be understood about how this transcription factor operates within the GA-response pathway. In order to isolate new components from the GA-response pathway, barley aleurone libraries were screened for GAMYB-binding proteins using a recently developed yeast two-hybrid system, which is...[Show more]
dc.contributor.author | Woodger, Fiona | |
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dc.contributor.author | Gubler, Frank | |
dc.contributor.author | Jacobsen, John | |
dc.contributor.author | Pogson, Barry | |
dc.date.accessioned | 2015-12-13T23:08:17Z | |
dc.identifier.issn | 0960-7412 | |
dc.identifier.uri | http://hdl.handle.net/1885/86609 | |
dc.description.abstract | GAMYB is a gibberellin (GA)-regulated activator of hydrolase gene expression in the aleurone layer of germinating cereal grains. Although it is clear that GAMYB expression is regulated by GA, more remains to be understood about how this transcription factor operates within the GA-response pathway. In order to isolate new components from the GA-response pathway, barley aleurone libraries were screened for GAMYB-binding proteins using a recently developed yeast two-hybrid system, which is compatible with the use of transcription factors as baits. We isolated a new member of the emerging Mak-subgroup of cdc2- and MAP kinase-related protein kinases. We have termed this GAMYB-binding protein KGM (for kinase associated with GAMYB). Transient expression of KGM specifically repressed α-amylase promoter activity at the level of GAMYB function but a mutation designed to de-stabilise the activation loop of KGM alleviated this repression. We propose that KGM is a negative regulator of GAMYB function in aleurone that may prevent precocious hydrolase gene expression. | |
dc.publisher | Blackwell Publishing Ltd | |
dc.source | The Plant Journal | |
dc.subject | Keywords: Genes; Grain (agricultural product); Mutagenesis; Proteins; Yeast; Hydrolase gene expression; Plants (botany); alpha-Amylase; Amino Acid Sequence; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Germination; Gibberellins; Horde a-amylase; Aleurone; GAMYB; Gibberellin; Mak; Signalling | |
dc.title | A Mak-like kinase is a repressor of GAMYB in barley aleurone | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 33 | |
dc.date.issued | 2003 | |
local.identifier.absfor | 060705 - Plant Physiology | |
local.identifier.ariespublication | MigratedxPub15524 | |
local.type.status | Published Version | |
local.contributor.affiliation | Woodger, Fiona, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Gubler, Frank , CSIRO | |
local.contributor.affiliation | Pogson, Barry, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Jacobsen, John, CSIRO Plant Industry | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 4 | |
local.bibliographicCitation.startpage | 707 | |
local.bibliographicCitation.lastpage | 717 | |
local.identifier.doi | 10.1046/j.1365-313X.2003.01663.x | |
dc.date.updated | 2015-12-12T08:12:57Z | |
local.identifier.scopusID | 2-s2.0-0037298311 | |
Collections | ANU Research Publications |
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