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NADPH Dehydrogenase-mediated respiratory electron transport in thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803 is inactive in the light

Ryu, Kwon-Sang; Suh, Kye-Hong; Chung, Young-Ho; Park, Young-Mok; Chow, Wah S (Fred); Park, Youn-Il

Description

Non-photochemical redox changes of the plastoquinone pools in darkness were investigated in the cyanobacterium Synechocystis sp. PCC 6803 by monitoring changes in Chl fluorescence yield during light-to-dark transitions. The inhibitors rotenone and mercury with or without 1 mM succinate fully suppressed the postillumination increase in Chl fluorescence in both NADPH dehydrogenase-defective (M55) and ΔCtaI cells. The latter cells lack subunit I of cytochrome aa3-type cytochrome c oxidase. These...[Show more]

dc.contributor.authorRyu, Kwon-Sang
dc.contributor.authorSuh, Kye-Hong
dc.contributor.authorChung, Young-Ho
dc.contributor.authorPark, Young-Mok
dc.contributor.authorChow, Wah S (Fred)
dc.contributor.authorPark, Youn-Il
dc.date.accessioned2015-12-13T23:08:06Z
dc.date.available2015-12-13T23:08:06Z
dc.identifier.issn1234-123x
dc.identifier.urihttp://hdl.handle.net/1885/86516
dc.description.abstractNon-photochemical redox changes of the plastoquinone pools in darkness were investigated in the cyanobacterium Synechocystis sp. PCC 6803 by monitoring changes in Chl fluorescence yield during light-to-dark transitions. The inhibitors rotenone and mercury with or without 1 mM succinate fully suppressed the postillumination increase in Chl fluorescence in both NADPH dehydrogenase-defective (M55) and ΔCtaI cells. The latter cells lack subunit I of cytochrome aa3-type cytochrome c oxidase. These results strongly suggest that NADPH dehydrogenase plays the major role in electron donation in the non-photo-chemical reduction of plastoquinone. The rising phase of post-illumination Chl fluorescence in both wild type pretreated with KCN, and ΔCtaI cells, was significantly slowed by low light illumination. We detected comparable photochemical levels of both photosystem (PS) II and PSI during steady state illumination in wild type and ΔCtaI cells. From these results, we suggest that respiratory electron flow involved in the non-photochemical redox change of plastoquinone is not likely to occur in the light.
dc.publisherKorean Society of Molecular and Cell Biology
dc.sourceMolecules and Cells
dc.subjectKeywords: reduced nicotinamide adenine dinucleotide phosphate dehydrogenase; cytochrome c oxidase; mercury; plastoquinone; rotenone; succinic acid; article; Cyanobacterium; electron transport; enzymology; light; physiology; spectrofluorometry; thylakoid; fluorescen Cytochrome aa3 Oxidase; Light Inactivation; NADPH Dehydrogenase; Synechocystis sp. PCC 6803
dc.titleNADPH Dehydrogenase-mediated respiratory electron transport in thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803 is inactive in the light
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume15
dc.date.issued2003
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationMigratedxPub15422
local.type.statusPublished Version
local.contributor.affiliationRyu, Kwon-Sang, Korea Research Institute of Standards and Science
local.contributor.affiliationSuh, Kye-Hong, Chungnam National University
local.contributor.affiliationChung, Young-Ho, Korea Basic Science Institute
local.contributor.affiliationPark, Young-Mok, Chungnam National University
local.contributor.affiliationChow, Wah S (Fred), College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPark, Youn-Il, Chungnam National University
local.bibliographicCitation.issue2
local.bibliographicCitation.startpage240
local.bibliographicCitation.lastpage244
dc.date.updated2015-12-12T08:12:16Z
local.identifier.scopusID2-s2.0-0038605066
CollectionsANU Research Publications

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