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Carbonic anhydrase and C4 photosynthesis a transgenic analysis

von Caemmerer, Susanne; Quinn, Vanda; Hancock, N; Price, Graeme (Dean); Furbank, Robert Thomas; Ludwig, M

Description

Carbonic anhydrase (CA, EC 4.2.1.1) catalyses the first reaction in the C4 photosynthetic pathway, the conversion of atmospheric CO 2 to bicarbonate in the mesophyll cytosol. To examine the importance of the enzyme to the functioning of the C4 photosynthetic pathway, Flaveria bidentis (L.) Kuntze, a C4 dicot, was genetically transformed with an antisense construct in which the cDNA encoding a putative cytosolic CA (CA3) was placed under the control of a constitutive promoter. Some of the...[Show more]

dc.contributor.authorvon Caemmerer, Susanne
dc.contributor.authorQuinn, Vanda
dc.contributor.authorHancock, N
dc.contributor.authorPrice, Graeme (Dean)
dc.contributor.authorFurbank, Robert Thomas
dc.contributor.authorLudwig, M
dc.date.accessioned2015-12-13T23:07:50Z
dc.date.available2015-12-13T23:07:50Z
dc.identifier.issn0140-7791
dc.identifier.urihttp://hdl.handle.net/1885/86380
dc.description.abstractCarbonic anhydrase (CA, EC 4.2.1.1) catalyses the first reaction in the C4 photosynthetic pathway, the conversion of atmospheric CO 2 to bicarbonate in the mesophyll cytosol. To examine the importance of the enzyme to the functioning of the C4 photosynthetic pathway, Flaveria bidentis (L.) Kuntze, a C4 dicot, was genetically transformed with an antisense construct in which the cDNA encoding a putative cytosolic CA (CA3) was placed under the control of a constitutive promoter. Some of the primary transformants had impaired CO2 assimilation rates and required high CO2 for growth. The T1 progeny of four primary transformants were used to examine the quantitative relationship between leaf CA activity and CO2 assimilation rate. CA activity was determined in leaf extracts with a mass spectrometric technique that measured the rate of 18O exchange from doubly labelled 13C 18O2. Steady-state CO2 assimilation rates were unaffected by a decrease in CA activity until CA activity was less than 20% of wild type when they decreased steeply. Transformants with less than 10% of wild-type CA activity had very low CO2 assimilation rates and grew poorly at ambient CO2 partial pressure. Reduction in CA activity also increased the CO2 partial pressure required to saturate CO 2 assimilation rates. The present data show that CA activity is essential for the functioning of the C4 photosynthetic pathway.
dc.publisherBlackwell Publishing Ltd
dc.sourcePlant Cell and Environment
dc.subjectKeywords: photosynthesis; Flaveria; Flaveria bidentis Antisense RNA; C4 photosynthesis; Carbonic anhydrase; Flaveria bidentis
dc.titleCarbonic anhydrase and C4 photosynthesis a transgenic analysis
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume27
dc.date.issued2004
local.identifier.absfor060705 - Plant Physiology
local.identifier.ariespublicationMigratedxPub15248
local.type.statusPublished Version
local.contributor.affiliationvon Caemmerer, Susanne, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationQuinn, Vanda, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHancock, N, Macquarie University
local.contributor.affiliationPrice, Graeme (Dean), College of Medicine, Biology and Environment, ANU
local.contributor.affiliationFurbank, Robert Thomas, CSIRO Division of Plant Industry
local.contributor.affiliationLudwig, M, Macquarie University
local.bibliographicCitation.startpage697
local.bibliographicCitation.lastpage703
local.identifier.doi10.1111/j.1365-3040.2003.01157.x
dc.date.updated2015-12-12T08:10:59Z
local.identifier.scopusID2-s2.0-2642558157
CollectionsANU Research Publications

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