Skip navigation
Skip navigation

Eosinophil degranulation in the allergic lung of mice primarily occurs in the airway lumen

Clark, Kristopher; Simson, Ljubov; Newcombe, Nicole; Koskinen, Aulikki; Lee, Nicola J; Lee, James L; Dent, Lindsay; Matthaei, Klaus; Foster, Paul S

Description

Eosinophil degranulation is thought to play a pivotal role in the pathogenesis of allergic disorders. Although mouse models of allergic disorders have been used extensively to identify the contribution of eosinophils to disease, ultrastructural evidence of active granule disassembly has not been reported. In this investigation, we characterized the degree of eosinophil activation in the bone marrow, blood, lung tissue, and airways lumen [bronchoalveolar lavage fluid (BALF)] of...[Show more]

dc.contributor.authorClark, Kristopher
dc.contributor.authorSimson, Ljubov
dc.contributor.authorNewcombe, Nicole
dc.contributor.authorKoskinen, Aulikki
dc.contributor.authorLee, Nicola J
dc.contributor.authorLee, James L
dc.contributor.authorDent, Lindsay
dc.contributor.authorMatthaei, Klaus
dc.contributor.authorFoster, Paul S
dc.date.accessioned2015-12-13T23:07:43Z
dc.date.available2015-12-13T23:07:43Z
dc.identifier.issn0741-5400
dc.identifier.urihttp://hdl.handle.net/1885/86321
dc.description.abstractEosinophil degranulation is thought to play a pivotal role in the pathogenesis of allergic disorders. Although mouse models of allergic disorders have been used extensively to identify the contribution of eosinophils to disease, ultrastructural evidence of active granule disassembly has not been reported. In this investigation, we characterized the degree of eosinophil activation in the bone marrow, blood, lung tissue, and airways lumen [bronchoalveolar lavage fluid (BALF)] of ovalbumin-sensitized and aero-challenged wild-type and interleukin-5 transgenic mice. Degranulation was most prominent in and primarily compartmentalized to the airways lumen. Eosinophils released granule proteins by the process of piece-meal degranulation (PMD). Accordingly, recruitment and activation of eosinophils in the lung correlated with the detection of cell-free eosinophil peroxidase in BALF and with the induction of airways hyper-reactivity. As in previous studies with human eosinophils, degranulation of isolated mouse cells did not occur until after adherence to extracellular matrix. However, higher concentrations of exogenous stimuli appear to be required to trigger adherence and degranulation (piecemeal) of mouse eosinophils when compared with values reported for studies of human eosinophils. Thus, mouse eosinophils undergo PMD during allergic inflammation, and in turn, this process may contribute to pathogenesis. However, the degranulation process in the allergic lung of mice is primarily compartmentalized to the airway lumen. Understanding the mechanism of eosinophil degranulation in the airway lumen may provide important insights into how this process occurs in human respiratory diseases.
dc.publisherFederation of American Societies for Experimental Biology
dc.sourceJournal of Leukocyte Biology
dc.subjectKeywords: eosinophil peroxidase; interleukin 5; major basic protein; ovalbumin; airway; allergic asthma; animal cell; animal model; article; asthma; bronchus hyperreactivity; cell activation; controlled study; eosinophil; immune response; lung disease; male; mouse; Asthma; Cellular activation; Eosinophil
dc.titleEosinophil degranulation in the allergic lung of mice primarily occurs in the airway lumen
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume75
dc.date.issued2004
local.identifier.absfor110701 - Allergy
local.identifier.ariespublicationMigratedxPub15158
local.type.statusPublished Version
local.contributor.affiliationClark, Kristopher, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSimson, Ljubov, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationNewcombe, Nicole, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationKoskinen, Aulikki, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLee, Nicola J, Garvan Institute of Medical Research
local.contributor.affiliationLee, James L, Mayo Clinic
local.contributor.affiliationDent, Lindsay, University of Adelaide
local.contributor.affiliationMatthaei, Klaus, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationFoster, Paul S, College of Medicine, Biology and Environment, ANU
local.bibliographicCitation.startpage1001
local.bibliographicCitation.lastpage1009
local.identifier.doi10.1189/jlb.0803391
dc.date.updated2015-12-12T08:10:20Z
local.identifier.scopusID2-s2.0-2642541824
CollectionsANU Research Publications

Download

There are no files associated with this item.


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator