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Differentiation of Embryonic Stem Cells into Functional Neurons In Vitro

Baharvand, Hossein; Power, John; Ozsarac, Nesrin; Matthaei, Klaus

Description

Embryonic stem (ES) cells are pluripotent cells that are derived from the inner cell mass of blastocysts. We produced neuronal cells by directed differentiation of a newly established murine ES cell line in vitro (Royan B1). Immunohistochemistry, scanning electron microscopy, Campenot's assay and electrophysiology were used to evaluate the differentiated neurons. Antibodies against microtubule-associated protein (MAP2), glutamic acid decarboxylase (GAD) and tyrosine hydroxylase (TH) antigens...[Show more]

dc.contributor.authorBaharvand, Hossein
dc.contributor.authorPower, John
dc.contributor.authorOzsarac, Nesrin
dc.contributor.authorMatthaei, Klaus
dc.date.accessioned2015-12-13T23:07:37Z
dc.identifier.issn0893-6609
dc.identifier.urihttp://hdl.handle.net/1885/86288
dc.description.abstractEmbryonic stem (ES) cells are pluripotent cells that are derived from the inner cell mass of blastocysts. We produced neuronal cells by directed differentiation of a newly established murine ES cell line in vitro (Royan B1). Immunohistochemistry, scanning electron microscopy, Campenot's assay and electrophysiology were used to evaluate the differentiated neurons. Antibodies against microtubule-associated protein (MAP2), glutamic acid decarboxylase (GAD) and tyrosine hydroxylase (TH) antigens showed that the cells had GABAergic and dopaminergic characteristics. The Campenot's assay revealed there were a number of peripheral nerveous system related neurons but they were rare. The cells also produced action potentials and responded to electrical stimulation by expressing calcium channels in the processes. We showed therefore that functional neurons can be generated by directed differentiation from embryonic stem cells in vitro.
dc.publisherJohn Wiley & Sons Inc
dc.sourceNeuroscience Research Communications
dc.titleDifferentiation of Embryonic Stem Cells into Functional Neurons In Vitro
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume35
dc.date.issued2004
local.identifier.absfor060410 - Neurogenetics
local.identifier.ariespublicationMigratedxPub15114
local.type.statusPublished Version
local.contributor.affiliationBaharvand, Hossein, Royan Institute Tehran
local.contributor.affiliationPower, John, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationOzsarac, Nesrin, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMatthaei, Klaus, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue2
local.bibliographicCitation.startpage130
local.bibliographicCitation.lastpage138
local.identifier.doi10.1002/nrc.20027
dc.date.updated2015-12-12T08:09:59Z
local.identifier.scopusID2-s2.0-9144239853
CollectionsANU Research Publications

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