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Immune response in rabbit ovaries following infection of a recombinant myxoma virus expressing rabbit zona pellucida protein B

Gu, Wenyi; Holland, Michael; Janssens, Peter; Seamark, Robert; Kerr, Peter

Description

In this study, we investigated the autoimmune response in rabbit ovaries following infection with a recombinant myxoma virus expressing rabbit zona pellucida protein B (MV-ZPB). A specific IgG antibody response to ZPB was elicited in the serum of infected rabbits and the antibody strongly bound to the zona pellucida of oocytes in secondary and tertiary follicles. T cell infiltration in the ovary was detected in a small proportion of the infected rabbits. In spite of this, the mean number of...[Show more]

dc.contributor.authorGu, Wenyi
dc.contributor.authorHolland, Michael
dc.contributor.authorJanssens, Peter
dc.contributor.authorSeamark, Robert
dc.contributor.authorKerr, Peter
dc.date.accessioned2015-12-13T23:07:36Z
dc.identifier.issn0042-6822
dc.identifier.urihttp://hdl.handle.net/1885/86284
dc.description.abstractIn this study, we investigated the autoimmune response in rabbit ovaries following infection with a recombinant myxoma virus expressing rabbit zona pellucida protein B (MV-ZPB). A specific IgG antibody response to ZPB was elicited in the serum of infected rabbits and the antibody strongly bound to the zona pellucida of oocytes in secondary and tertiary follicles. T cell infiltration in the ovary was detected in a small proportion of the infected rabbits. In spite of this, the mean number of preovulatory and tertiary follicles in the ovary was significantly reduced at 30 days postinfection compared with that of the infected and uninfected controls. Histological analysis revealed that the cortex and medulla of these ovaries had accumulated a large number of probably luteinized cells and there were no follicles in these areas, indicating the ovaries were in a severe pathological condition. The data suggest that the delivery of ZP antigens using a recombinant myxoma virus is a prospective way to develop immunocontraceptive vaccines for rabbit population control, but that more understanding of the kinetics of the autoimmune response induced by viral delivery is needed.
dc.publisherAcademic Press
dc.sourceVirology
dc.subjectKeywords: immunoglobulin G antibody; unclassified drug; virus protein; zona pellucida protein B; animal cell; animal experiment; animal model; animal tissue; antibody response; article; cell infiltration; controlled study; histology; immune response; luteinization; Immunocontraception; Ovary; Rabbit ZPB; Recombinant myxoma virus
dc.titleImmune response in rabbit ovaries following infection of a recombinant myxoma virus expressing rabbit zona pellucida protein B
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume318
dc.date.issued2004
local.identifier.absfor060506 - Virology
local.identifier.ariespublicationMigratedxPub15110
local.type.statusPublished Version
local.contributor.affiliationGu, Wenyi, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHolland, Michael, CSIRO
local.contributor.affiliationJanssens, Peter, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationSeamark, Robert, Flinders University
local.contributor.affiliationKerr, Peter, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue2
local.bibliographicCitation.startpage516
local.bibliographicCitation.lastpage523
local.identifier.doi10.1016/j.virol.2003.10.021
dc.date.updated2015-12-12T08:09:57Z
local.identifier.scopusID2-s2.0-1242283915
CollectionsANU Research Publications

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