Skip navigation
Skip navigation

T Helper-2 Immunity Regulates Bronchial Hyperresponsiveness in Eosinophil-Associated Gastrointestinal Disease in Mice

Forbes, Elizabeth; Prescott, Vanessa; D'Aprile, Angela; Henry, Peter J.; Yang, Ming; Matthaei, Klaus; Rothenberg, Marc E; Foster, Paul S; Hogan, Simon

Description

Background & Aims: Eosinophil-associated gastrointestinal diseases are frequently associated with extraintestinal features, including bronchopulmonary manifestations. The factors predisposing to bronchial hyperresponsiveness in eosinophil-associated gastrointestinal diseases are unknown. To elucidate the mechanistic link between eosinophil-associated gastrointestinal diseases and bronchial hyperresponsiveness, we used murine models of eosinophil-associated gastrointestinal diseases and...[Show more]

dc.contributor.authorForbes, Elizabeth
dc.contributor.authorPrescott, Vanessa
dc.contributor.authorD'Aprile, Angela
dc.contributor.authorHenry, Peter J.
dc.contributor.authorYang, Ming
dc.contributor.authorMatthaei, Klaus
dc.contributor.authorRothenberg, Marc E
dc.contributor.authorFoster, Paul S
dc.contributor.authorHogan, Simon
dc.date.accessioned2015-12-13T23:07:35Z
dc.identifier.issn0016-5085
dc.identifier.urihttp://hdl.handle.net/1885/86275
dc.description.abstractBackground & Aims: Eosinophil-associated gastrointestinal diseases are frequently associated with extraintestinal features, including bronchopulmonary manifestations. The factors predisposing to bronchial hyperresponsiveness in eosinophil-associated gastrointestinal diseases are unknown. To elucidate the mechanistic link between eosinophil-associated gastrointestinal diseases and bronchial hyperresponsiveness, we used murine models of eosinophil-associated gastrointestinal diseases and eotaxin-1/transgene-induced eosinophil-associated gastrointestinal diseases. Methods: Mice were sensitized and orally challenged with ovalbumin-coated encapsulated particles to induce eosinophil-associated gastrointestinal disease, and bronchial responsiveness was examined. Furthermore, transgenic mice expressing eotaxin in the intestine (with the rat fatty acid-binding promoter) were used to specifically elucidate the contribution of this chemokine in eosinophil-associated gastrointestinal disease-associated bronchial hyperresponsiveness. Results: The induction of allergen-induced eosinophil-associated gastrointestinal disease was directly correlated with the development of bronchial hyperresponsiveness. The development of bronchial hyperresponsiveness in mice with allergen-induced eosinophil-associated gastrointestinal disease was dependent on eotaxin expression in the gastrointestinal tract. Expression of eotaxin in the gastrointestinal tract of transgenic mice was sufficient to promote bronchial hyperresponsiveness. Bronchial hyperresponsiveness was shown to be directly linked to the aberrant CD4+ T helper 2 lymphocyte production of interleukin-13. It is interesting to note that transgenic expression of eotaxin was linked with enhanced T helper 2 lymphocyte/cytokine synthesis (interleukin-4, -5, and -13) and the production of mucosal immunoglobulin G1 in the gastrointestinal lumen. We also showed that eotaxin treatment of CD4+ T cells enhanced interleukin-13 production in vitro. Conclusions: These studies suggest that increased expression of eotaxin in the gastrointestinal compartment can lead to increased CD4+ T cell-derived T helper 2 lymphocyte-cytokine production that drives aberrant immunophysiological responses in distant noninflamed mucosal tissue (the lung). These results provide a possible explanation for the altered lung function seen in some patients with inflammatory gastrointestinal disorders.
dc.publisherW B Saunders Co
dc.sourceGastroenterology
dc.subjectKeywords: allergen; CD4 antigen; chemokine; eotaxin; fatty acid; immunoglobulin G1; interleukin 13; interleukin 4; interleukin 5; ovalbumin; animal experiment; animal model; animal tissue; article; bronchus hyperreactivity; cellular immunity; controlled study; cyto allophycocyanin; APC; BHR; bronchial hyperresponsiveness; CCR3; chemokine receptor-3; EGID; ELISA; enzyme-linked immunosorbent assay; eosinophil-associated gastrointestinal diseases; FCS; fetal calf serum; gastrointestinal; GI; IFABPp
dc.titleT Helper-2 Immunity Regulates Bronchial Hyperresponsiveness in Eosinophil-Associated Gastrointestinal Disease in Mice
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume127
dc.date.issued2004
local.identifier.absfor110701 - Allergy
local.identifier.ariespublicationMigratedxPub15098
local.type.statusPublished Version
local.contributor.affiliationForbes, Elizabeth, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationPrescott, Vanessa, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationD'Aprile, Angela, University of Western Australia
local.contributor.affiliationHenry, Peter J., University of Western Australia
local.contributor.affiliationYang, Ming, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationMatthaei, Klaus, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRothenberg, Marc E, University of Cincinnati
local.contributor.affiliationFoster, Paul S, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationHogan, Simon, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage105
local.bibliographicCitation.lastpage118
local.identifier.doi10.1053/j.gastro.2004.03.057
dc.date.updated2015-12-12T08:09:52Z
local.identifier.scopusID2-s2.0-3242720765
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Forbes_T_Helper-2_Immunity_Regulates_2004.pdf355.17 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator