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RNA interference demonstrates a novel role for H2A.Z in chromosome segregation

Rangasamy, Danny; Greaves, Ian; Tremethick, David

Description

The histone variant H2A.Z plays an essential role in metazoans but its function remains to be determined. Here, we developed a new inducible RNAi strategy to elucidate the role of H2A.Z in mammalian cell lines. We show that in the absence of H2A.Z, the genome becomes highly unstable and that this instability is caused by defects in the chromosome segregation process. Analysis of H2A.Z localization reveals that in these cells it is enriched at heterochromatic foci with HP1α on the arms of...[Show more]

dc.contributor.authorRangasamy, Danny
dc.contributor.authorGreaves, Ian
dc.contributor.authorTremethick, David
dc.date.accessioned2015-12-13T23:06:58Z
dc.identifier.issn1545-9993
dc.identifier.urihttp://hdl.handle.net/1885/86010
dc.description.abstractThe histone variant H2A.Z plays an essential role in metazoans but its function remains to be determined. Here, we developed a new inducible RNAi strategy to elucidate the role of H2A.Z in mammalian cell lines. We show that in the absence of H2A.Z, the genome becomes highly unstable and that this instability is caused by defects in the chromosome segregation process. Analysis of H2A.Z localization reveals that in these cells it is enriched at heterochromatic foci with HP1α on the arms of chromosomes but not at centromeric regions. When H2A.Z is depleted, normal HP1α-chromatin interactions are disrupted on the chromosomal arms and, notably, also at pericentric regions. Therefore, H2A.Z controls the localization of HP1α. We conclude that H2A.Z is essential for the accurate transmission of chromosomes.
dc.publisherNature Publishing Group
dc.sourceNature Structural and Molecular Biology
dc.subjectKeywords: histone H2A; histone H2A.Z; RNA; unclassified drug; animal cell; article; cell line; centromere; chromatin structure; chromosomal parameters; chromosome aberration; chromosome segregation; chromosome structure; embryo; gene function; genome; heterochromat
dc.titleRNA interference demonstrates a novel role for H2A.Z in chromosome segregation
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume11
dc.date.issued2004
local.identifier.absfor060199 - Biochemistry and Cell Biology not elsewhere classified
local.identifier.ariespublicationMigratedxPub14733
local.type.statusPublished Version
local.contributor.affiliationRangasamy, Danny, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationGreaves, Ian, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationTremethick, David, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue7
local.bibliographicCitation.startpage650
local.bibliographicCitation.lastpage655
local.identifier.doi10.1038/nsmb786
dc.date.updated2015-12-12T08:07:26Z
local.identifier.scopusID2-s2.0-3042642001
CollectionsANU Research Publications

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