Skip navigation
Skip navigation

H2A.Z Alters the Nucleosome Surface to Promote HP1�-Mediated Chromatin Fiber Folding

Fan, Jun; Rangasamy, Danny; Luger, Karolin; Tremethick, David

Description

Controlling the degree of higher order chromatin folding is a key element in partitioning the metazoan genome into functionally distinct chromosomal domains. However, the mechanism of this fundamental process is poorly understood. Our recent studies suggested that the essential histone variant H2A.Z and the silencing protein HP1α may function together to establish a specialized conformation at constitutive heterochromatic domains. We demonstrate here that HP1α is a unique chromatin binding...[Show more]

dc.contributor.authorFan, Jun
dc.contributor.authorRangasamy, Danny
dc.contributor.authorLuger, Karolin
dc.contributor.authorTremethick, David
dc.date.accessioned2015-12-13T23:06:21Z
dc.identifier.issn1097-2765
dc.identifier.urihttp://hdl.handle.net/1885/85990
dc.description.abstractControlling the degree of higher order chromatin folding is a key element in partitioning the metazoan genome into functionally distinct chromosomal domains. However, the mechanism of this fundamental process is poorly understood. Our recent studies suggested that the essential histone variant H2A.Z and the silencing protein HP1α may function together to establish a specialized conformation at constitutive heterochromatic domains. We demonstrate here that HP1α is a unique chromatin binding protein. It prefers to bind to condensed higher order chromatin structures and alters the chromatin-folding pathway in a novel way to locally compact individual chromatin fibers without crosslinking them. Strikingly, both of these features are enhanced by an altered nucleosomal surface created by H2A.Z (the acidic patch). This shows that the surface of the nucleosome can regulate the formation of distinct higher order chromatin structures mediated by an architectural chromatin binding protein.
dc.publisherCell Press
dc.sourceMolecular Cell
dc.titleH2A.Z Alters the Nucleosome Surface to Promote HP1�-Mediated Chromatin Fiber Folding
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume16
dc.date.issued2004
local.identifier.absfor060199 - Biochemistry and Cell Biology not elsewhere classified
local.identifier.ariespublicationMigratedxPub14706
local.type.statusPublished Version
local.contributor.affiliationFan, Jun, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationRangasamy, Danny, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationLuger, Karolin, Colorado State University
local.contributor.affiliationTremethick, David, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage655
local.bibliographicCitation.lastpage661
local.identifier.doi10.1016/j.molcel.2004.10.023
dc.date.updated2015-12-12T08:04:53Z
local.identifier.scopusID2-s2.0-8844281536
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Fan_H2A.Z_Alters_the_Nucleosome_2004.pdf309.72 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator