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Angular dependence of dipole-dipole-Curie-spin cross-correlation effects in high-spin and low-spin paramagnetic myoglobin

Pintacuda, Guido; Hohenthanner, Karin; Norbert, M; Otting, Gottfried

Description

The 15N-HSQC spectra of low-spin cyano-met-myoglobin and high-spin fluoro-met-myoglobin were assigned and dipole-dipole-Curie-spin cross-correlated relaxation rates measured. These cross-correlation rates originating from the dipolar 1H-15N interaction and the dipolar interaction between the 1H and the Curie spin of the paramagnetic center contain long-range angular information about the orientation of the 1H-15N bond with respect to the iron-1H vector, with information measurable up to 11 Å...[Show more]

dc.contributor.authorPintacuda, Guido
dc.contributor.authorHohenthanner, Karin
dc.contributor.authorNorbert, M
dc.contributor.authorOtting, Gottfried
dc.date.accessioned2015-12-13T23:06:04Z
dc.date.available2015-12-13T23:06:04Z
dc.identifier.issn0925-2738
dc.identifier.urihttp://hdl.handle.net/1885/85846
dc.description.abstractThe 15N-HSQC spectra of low-spin cyano-met-myoglobin and high-spin fluoro-met-myoglobin were assigned and dipole-dipole-Curie-spin cross-correlated relaxation rates measured. These cross-correlation rates originating from the dipolar 1H-15N interaction and the dipolar interaction between the 1H and the Curie spin of the paramagnetic center contain long-range angular information about the orientation of the 1H-15N bond with respect to the iron-1H vector, with information measurable up to 11 Å from the metal for the low-spin complex, and between 10 to 25 Å for the high-spin complex. Comparison of the experimental data with predictions from crystal structure data showed that the anisotropy of the magnetic susceptibility tensor in low spin cyano-met-myoglobin significantly influences the cross-correlated dipole-dipole-Curie-spin relaxation rates.
dc.publisherKluwer Academic Publishers
dc.sourceJournal of Biomolecular NMR
dc.subjectKeywords: hydrogen; metal; metmyoglobin; nitrogen 15; anisotropy; article; chemical bond; controlled study; crystal structure; dipole; magnetism; mathematical analysis; molecular interaction; nonhuman; nuclear magnetic resonance spectroscopy; prediction; priority j Anistropy of magnetic susceptibility; Dipole-dipole-Curie-spin cross corelation; Long-range angle restraints; Magnetic susceptibility tensor; Paramagnetic relaxation; Sperm-whale myoglobin
dc.titleAngular dependence of dipole-dipole-Curie-spin cross-correlation effects in high-spin and low-spin paramagnetic myoglobin
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume27
dc.date.issued2003
local.identifier.absfor030101 - Analytical Spectrometry
local.identifier.ariespublicationMigratedxPub14513
local.type.statusPublished Version
local.contributor.affiliationPintacuda, Guido, Ecole Normale Superieure de Lyon
local.contributor.affiliationHohenthanner, Karin, Johannes Kepler University Linz
local.contributor.affiliationOtting, Gottfried, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationM, Norbert, Johannes Kepler University Linz
local.bibliographicCitation.issue2
local.bibliographicCitation.startpage115
local.bibliographicCitation.lastpage132
local.identifier.doi10.1023/A:1024926126239
dc.date.updated2015-12-12T08:03:29Z
local.identifier.scopusID2-s2.0-0042387719
CollectionsANU Research Publications

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