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Micromanipulation of phospholipid bilayers by atomic force microscopy

Maeda, Nobuo; Senden, Timothy; Di Meglio, Jean-Marc

Description

The molecular details of adhesion mechanics in phospholipid bilayers have been studied using atomic force microscopy (AFM). Under tension fused bilayers of dipalmitoylphosphatidylcholine (DPPC) yield to give non-distance dependent and discrete force plateaux of 45.4, 81.6 and 113±3.5 pN. This behaviour may persist over distances as great as 400 nm and suggests the stable formation of a cylindrical tube which bridges the bilayers on the two surfaces. The stability of this connective structure...[Show more]

dc.contributor.authorMaeda, Nobuo
dc.contributor.authorSenden, Timothy
dc.contributor.authorDi Meglio, Jean-Marc
dc.date.accessioned2015-12-13T23:05:52Z
dc.identifier.issn0005-2736
dc.identifier.urihttp://hdl.handle.net/1885/85751
dc.description.abstractThe molecular details of adhesion mechanics in phospholipid bilayers have been studied using atomic force microscopy (AFM). Under tension fused bilayers of dipalmitoylphosphatidylcholine (DPPC) yield to give non-distance dependent and discrete force plateaux of 45.4, 81.6 and 113±3.5 pN. This behaviour may persist over distances as great as 400 nm and suggests the stable formation of a cylindrical tube which bridges the bilayers on the two surfaces. The stability of this connective structure may have implications for the formation of pili and hence for the initial stage of bacterial conjugation. Dimyristoylphosphatidylcholine (DMPC) bilayers also exhibit force plateaux but with a much less pronounced quantization. Bilayers composed of egg PC, sterylamine and cholesterol stressed in a similar way show complex behaviour which can in part be explained using the models demonstrated in the pure lipids.
dc.publisherElsevier
dc.sourceBiochimica et Biophysica Acta: Biomembranes
dc.subjectKeywords: amine; cholesterol; dimyristoylphosphatidylcholine; dipalmitoylphosphatidylcholine; phosphatidylcholine; sterylamine; unclassified drug; article; atomic force microscopy; bacterium adherence; bacterium pilus; complex formation; lipid analysis; molecular m Atomic force microscopy; Bilayer; Membrane fusion; Phospholipids; Pilus; Tubule; Vesicle
dc.titleMicromanipulation of phospholipid bilayers by atomic force microscopy
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume1564
dc.date.issued2002
local.identifier.absfor020201 - Atomic and Molecular Physics
local.identifier.ariespublicationMigratedxPub14396
local.type.statusPublished Version
local.contributor.affiliationMaeda, Nobuo, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationSenden, Timothy , College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationDi Meglio, Jean-Marc, Physicio-Chimique
local.description.embargo2037-12-31
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage165
local.bibliographicCitation.lastpage172
local.identifier.doi10.1016/S0005-2736(02)00443-1
dc.date.updated2015-12-12T08:02:45Z
local.identifier.scopusID2-s2.0-0037136064
CollectionsANU Research Publications

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