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An efficient system for high-level expression and easy purification of authentic recombinant proteins

Catanzariti, Ann-Maree; Soboleva, Tatiana; Jans, David A; Board, Philip; Baker, Rohan


Expression of recombinant proteins as fusions to the eukaryotic protein ubiquitin has been found to significantly increase the yield of unstable or poorly expressed proteins. The benefit of this technique is further enhanced by the availability of naturally occurring deubiquitylating enzymes, which remove ubiquitin from the fusion product. However, the versatility of the system has been constrained due to the lack of a robust, easily purified deubiquitylating enzyme. Here we report the...[Show more]

CollectionsANU Research Publications
Date published: 2004
Type: Journal article
Source: Protein Science
DOI: 10.1110/ps.04618904


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