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Lanthanide labeling offers fast NMR approach to 3D structure determinations of protein-protein complexes

Pintacuda, Guido; Park, Ah Young; Keniry, Max; Dixon, Nicholas; Otting, Gottfried


A novel nuclear magnetic resonance (NMR) strategy based on labeling with lanthanides achieves rapid determinations of accurate three-dimensional (3D) structures of protein-protein complexes. The method employs pseudocontact shifts (PCS) induced by a site-specifically bound lanthanide ion to anchor the coordinate system of the magnetic susceptibility tensor in the molecular frames of the two molecules. Simple superposition of the tensors detected in the two protein molecules brings them together...[Show more]

CollectionsANU Research Publications
Date published: 2006
Type: Journal article
Source: Journal of the American Chemical Society
DOI: 10.1021/ja057008z


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