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Protective immunity against secondary poxvirus infection is dependent on antibody but not on CD4 or CD8 T-cell function

Panchanathan, Vijay; Chaudhri, Geeta; Karupiah, Gunasegaran

Description

Renewed interest in smallpox and the need for safer vaccines have highlighted our lack of understanding of the requirements for protective immunity. Since smallpox has been eradicated, surrogate animal models of closely related orthopoxviruses, such as ectromelia virus, have been used to establish critical roles for CD8 T cells in the control of primary infection. To study the requirements for protection against secondary infection, we have used a prime-challenge regime, in which avirulent...[Show more]

dc.contributor.authorPanchanathan, Vijay
dc.contributor.authorChaudhri, Geeta
dc.contributor.authorKarupiah, Gunasegaran
dc.date.accessioned2015-12-13T23:04:01Z
dc.identifier.issn0022-538X
dc.identifier.urihttp://hdl.handle.net/1885/85185
dc.description.abstractRenewed interest in smallpox and the need for safer vaccines have highlighted our lack of understanding of the requirements for protective immunity. Since smallpox has been eradicated, surrogate animal models of closely related orthopoxviruses, such as ectromelia virus, have been used to establish critical roles for CD8 T cells in the control of primary infection. To study the requirements for protection against secondary infection, we have used a prime-challenge regime, in which avirulent ectromelia virus was used to prime mice that were then challenged with virulent ectromelia virus. In contrast to primary infection, T cells are not required for recovery from secondary infection, since gene knockout mice deficient in CD8 T-cell function and wild-type mice acutely depleted of CD4, CD8, or both subsets were fully protected. Protection correlated with effective virus control and generation of neutralizing antibody. Notably, primed mice that lacked B cells, major histocompatibility complex class II, or CD40 succumbed to secondary infection. Thus, antibody is essential, but CD4 or CD8 T cells are not required for recovery from secondary poxvirus infection.
dc.publisherAmerican Society for Microbiology
dc.sourceJournal of Virology
dc.subjectKeywords: CD40 antigen; major histocompatibility antigen class 2; neutralizing antibody; virus vaccine; animal cell; animal experiment; antibody production; article; B lymphocyte; CD4+ T lymphocyte; CD8+ T lymphocyte; controlled study; Ectromelia virus; female; kno
dc.titleProtective immunity against secondary poxvirus infection is dependent on antibody but not on CD4 or CD8 T-cell function
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume80
dc.date.issued2006
local.identifier.absfor110799 - Immunology not elsewhere classified
local.identifier.ariespublicationMigratedxPub13445
local.type.statusPublished Version
local.contributor.affiliationPanchanathan, Vijay, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationChaudhri, Geeta, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationKarupiah, Gunasegaran, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue13
local.bibliographicCitation.startpage6333
local.bibliographicCitation.lastpage6338
local.identifier.doi10.1128/JVI.00115-06
dc.date.updated2015-12-12T07:52:56Z
local.identifier.scopusID2-s2.0-33745228965
CollectionsANU Research Publications

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