15 N-Labelled proteins by cell-free protein synthesis. Strategies for high-throughput NMR studies of proteins and protein-ligand complexes
[15N]-heteronuclear single quantum coherence (HSQC) spectra provide a readily accessible fingerprint of [15N]-labelled proteins, where the backbone amide group of each nonproline amino acid residue contributes a single cross-peak. Cell-free protein synthesis offers a fast and economical route to enhance the information content of [15N]-HSQC spectra by amino acid type selective [15N]-labelling. The samples can be measured without chromatographic protein purification, dilution of isotopes by...[Show more]
|Collections||ANU Research Publications|
|Source:||The FEBS Journal|
|01_Ozawa_15_N-Labelled_proteins_by_2006.pdf||1.18 MB||Adobe PDF||Request a copy|
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