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The purification, crystallization and preliminary diffraction of a glycerophosphodiesterase from Enterobacter aerogenes

Jackson, Colin; Carr, Paul D; Kim, Hye-Kyung; Liu, Jian-Wei; Ollis, David


The metallo-glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) has been cloned, expressed in Escherichia coli and purified. Initial screening of crystallization conditions for this enzyme resulted in the identification of needles from one condition in a sodium malonate grid screen. Removal of the metals from the enzyme and subsequent optimization of these conditions led to crystals that diffracted to 2.9 Å and belonged to space group P2 13, with unit-cell parameter a = 164.1 Å....[Show more]

CollectionsANU Research Publications
Date published: 2006
Type: Journal article
Source: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
DOI: 10.1107/S1744309106020021


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