Jackson, Colin; Carr, Paul D; Kim, Hye-Kyung; Liu, Jian-Wei; Ollis, David
The metallo-glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) has been cloned, expressed in Escherichia coli and purified. Initial screening of crystallization conditions for this enzyme resulted in the identification of needles from one condition in a sodium malonate grid screen. Removal of the metals from the enzyme and subsequent optimization of these conditions led to crystals that diffracted to 2.9 Å and belonged to space group P2 13, with unit-cell parameter a = 164.1 Å....[Show more]
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