Cheung, Timothy; Hearn, John
Dimerization between wallaby GnRH-R and its splice variants was examined. A baculovirus-based fluorescence resonance energy transfer (Bv-FRET) assay was used to assess protein-protein interaction between wild type wallaby GnRH-R and splice variants (GnRH-RΔ1 and GnRH-RΔ2). FRET analysis demonstrated that GnRH-R, GnRH-RΔ1 or GnRH-RΔ2 are capable of assembling as homodimers. When GnRH-R is co-expressed with GnRH-RΔ1 or GnRH-RΔ2 splice variants, GnRH-R can form heterodimers with GnRH-RΔ1 and...[Show more]
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