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Short tandem repeat (STR) genotyping of keratinised hair Part 2. An optimised genomic DNA extraction procedure reveals donor dependence of STR profiles

McNevin, Dennis; Wilson-Wilde, Linzi; Robertson, James; Kyd, Jennelle M; Lennard, Christopher James

Description

A feasibility study of short tandem repeat (STR) genotyping of telogen phase hairs in particular, and hair shaft in general, is presented. A number of extraction procedures in common use were investigated and the quantities of nuclear DNA (nuDNA) delivered were quantified via a real-time PCR assay. The extracts were subjected to two variations on AmpFℓSTR® Profiler Plus™ PCR amplification strategies (extended cycles, two rounds of PCR) and the genotypes compared. Nuclear DNA was found to...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
URI: http://hdl.handle.net/1885/84195
Source: Forensic Science International
DOI: 10.1016/j.forsciint.2005.05.005

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