Graham, Stephen C; Lilley, Penelope; Lee, Mihwa; Schaeffer, Patrick; Kralicek, Andrew V; Dixon, Nicholas; Guss, Jules Mitchell
Aminopeptidase P (APPro) is a manganese-dependent enzyme that cleaves the N-terminal amino acid from polypeptides where the second residue is proline. APPro shares a similar fold, substrate specificity, and catalytic mechanism with methionine aminopeptidase and prolidase. To investigate the roles of conserved residues at the active site, seven mutant forms of APPro were characterized kinetically and structurally. Mutation of individual metal ligands selectively abolished binding of either or...[Show more]
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.