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Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: insights into substrate recognition and the mechanism of catalysis

Graham, Stephen C; Lilley, Penelope; Lee, Mihwa; Schaeffer, Patrick; Kralicek, Andrew V; Dixon, Nicholas; Guss, Jules Mitchell


Aminopeptidase P (APPro) is a manganese-dependent enzyme that cleaves the N-terminal amino acid from polypeptides where the second residue is proline. APPro shares a similar fold, substrate specificity, and catalytic mechanism with methionine aminopeptidase and prolidase. To investigate the roles of conserved residues at the active site, seven mutant forms of APPro were characterized kinetically and structurally. Mutation of individual metal ligands selectively abolished binding of either or...[Show more]

CollectionsANU Research Publications
Date published: 2006
Type: Journal article
Source: Biochemistry
DOI: 10.1021/bi0518904


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